|
KMID : 0604520080340020117
|
|
Journal of the Society of Cosmetic Scientists of Korea
2008 Volume.34 No. 2 p.117 ~ p.127
|
|
|
Antioxidative Activity and Component Analysis of Cayratia japonica Extract
|
|
Yang Hee-Jung
Kim Eun-Hee
Park Soo-Nam
|
|
|
Abstract
|
|
|
|
In this study, the antioxidative effects, inhibitory effects on elastase, and components of Cayratia japonica extracts were investigated. The free radical(1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities() of extract/fractions of Cayratia japonica were in the order: 50% ethanol extract(114.3 ))). Reactive oxygen species(ROS) scavenging activities() of some Cayratia japonica extracts in system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activities were deglycosylated flavonoid aglycone fraction()<50% ethanol extract(1.21 )). Ethyl acetate fraction showed the most prominent scavenging activity. The protective effects of extract/fractions of Cayratia japonica on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Cayratia japonica extracts suppressed photohemolysis in a concentration dependent manner(), particularly deglycosylated flavonoid aglycone fraction exhibited the most prominent celluar protective effect(, 175.05min at 25 ). Aglycone fractions obtained from the deglycosylation reaction of ethyl acetate fraction among the Cayratia japonica extracts, showed 2 bands in TLC and 2 peaks in HPLC experiments(360 nm). Two components were identified as luteolin(composition ratio, 47.50%), apigenin(52.50). TLC chromatogram of ethyl acetate fraction of Cayratia japonica extract revealed 3 bands and HPLC chromatogram showed 4 peaks, which were identified as luteolin-7-O--D-glucopyranoside(composition ratio, 11.14%), apigenin-7-O--D-glucuronopyranoside(15.38%), luteolin(23.55%) and apigenin(49.92%) in the order of elution time. The inhibitory effect of aglycone fraction on elastase() was very high. These results indicate that extract/fractions of Cayratia japonica can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging and other ROS, and protect cellular membranes against ROS. And component analysis of Cayratia japonica extract and antioxidative effects could be applicable to new cosmetics.
|
|
|
KEYWORD
|
|
|
Cayratia japonica, flavonoid, antioxidative activity, elastase, cosmetics
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|